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Reports
Nek6 is involved in G2/M phase cell cycle arrest through DNA damage-induced phosphorylation
Min-Young Lee, Hyun-Ju Kim, Myoung-Ae Kim, Hye Jin Jee, Ae Jeong Kim, Yoe-Sik Bae, Joo-In Park, Jay H. Chung and Jeanho Yun
volume 7 | issue 17
1 September 2008Pages: 2705 - 2709
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Nek6 is a recently identified NIMA-related kinase that is required for mitotic cell cycle progression. In the present study, we examined the role of Nek6 in the DNA damage response. We found that Nek6 is phosphorylated upon IR and UV irradiation through the DNA damage checkpoint in vivo. Nek6 is also directly phosphorylated by the checkpoint kinases Chk1 and Chk2 in vitro. Notably, Nek6 activation during mitosis is completely abolished by IR and UV irradiation. Moreover, the ectopic expression of Nek6 overrides DNA damage-induced G2/M arrest. These results suggest that Nek6 is a novel target of the DNA damage checkpoint and that the inhibition of Nek6 activity is required for proper cell cycle arrest in the G2/M phase upon DNA damage.
Authors
Min-Young Lee
Dong-A University; Busan, South Korea;
Hyun-Ju Kim
Dong-A University; Busan, South Korea;
Myoung-Ae Kim
Dong-A University; Busan, South Korea;
Hye Jin Jee
Dong-A University; Busan, South Korea;
Ae Jeong Kim
Dong-A University; Busan, South Korea;
Yoe-Sik Bae
Dong-A University; Busan, South Korea;
Joo-In Park
Dong-A University; Busan, South Korea;
Jay H. Chung
National Institutes of Health; Maryland, USA
Jeanho Yun
Dong-A University; Busan, South Korea;