Sign up for Table of Contents Alerts.
Email this page
Print this page
Report
Novel C5a Agonist-Based Dendritic Cell Vaccine in a Murine Model of Melanoma
Anthony A. Floreani, Sandra J. Gunselman, Arthur J. Heires, Ralph J. Hauke, Stefano Tarantolo and John D. Jackson
volume 6 | issue 22
15 November 2007Pages: 2835 - 2839
Subscribe to this journal for $129/year
A novel method to improve targeting and presentation of poorly immunogenic tumor-related antigens was investigated. This was performed with a molecular adjuvant constructed by covalently linking a response selective peptide agonist of C5a (YSFKDMP(MeL)aR) to known melanoma tumor-related antigens. C57Bl/6J mice were injected subcutaneously with bone marrow derived dendritic cells (DCs) pulsed with a melanoma epitope (TRP2-P2/Agonist), melanoma epitope tyrosinase (TYR/Agonist), a nonfunctional reverse conformation C5a agonist bound to TYR(reverse peptide) or DMSO-PBS vehicle. Mice were injected with the pulsed DCs and cytokines IL-2 and GM-CSF three times prior to subcutaneous challenge with B16-F10 melanoma cells. All groups subsequently received DC vaccine boosters twice per week. Tumor growth was reduced and survival enhanced in mice immunized with the combination of TRP2-P2/Agonist and TYR/Agonist compared to mice receiving reverse peptide or vehicle.
Authors
Anthony A. Floreani
University of Nebraska Medical Center; Omaha, NE
Sandra J. Gunselman
University of Nebraska Medical Center; Omaha, NE
Arthur J. Heires
University of Nebraska Medical Center; Omaha, NE
Ralph J. Hauke
University of Nebraska Medical Center; Omaha, NE
Stefano Tarantolo
University of Nebraska Medical Center; Omaha, NE
John D. Jackson
University of Nebraska Medical Center; Omaha, NE