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Brief Report

Cell Cycle-Dependent Induction of Autophagy, Mitophagy and Reticulophagy

Ezgi Tasdemir, M. Chiara Maiuri, Nicolas Tajeddine, Ilio Vitale, Alfredo Criollo, José Miguel Vicencio, John A. Hickman, Olivier Geneste and Guido Kroemer

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When added to cells, a variety of autophagy inducers that operate through distinct mechanisms and target different organelles for autophagic destruction (mitochondria in mitophagy, endoplasmic reticulum in reticulophagy) rarely induce autophagic vacuolization in more than 50% or the cells. Here we show that this heterogeneity may be explained by cell cycle-specific effects. The BH3 mimetic ABT737, lithium, rapamycin, tunicamycin or nutrient depletion stereotypically induce autophagy preferentially in the G1 and S phases of the cell cycle, as determined by simultaneous monitoring of cell cycle markers and the cytoplasmic aggregation of GFP-LC3 in autophagic vacuoles. These results point to a hitherto neglected crosstalk between autophagic vacuolization and cell cycle regulation.

Authors

Ezgi Tasdemir

Institut Gustave Roussy

M. Chiara Maiuri

INSERM U848; Villejuif, France

Nicolas Tajeddine

Institut Gustave Roussy

Ilio Vitale

INSERM; Villejuif, France

Alfredo Criollo

Institut Gustave Roussy

José Miguel Vicencio

Institut Gustave Roussy

John A. Hickman

Institut de Recherche Servier

Olivier Geneste

Institut de Recherche Servier

Guido Kroemer

INSERM; Villejuif, France

Purchase article for $19

Subscribe to this journal for $129/year