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Methylation of Human MicroRNA Genes in Normal and Neoplastic Cells
Barbara Weber, Carlo Stresemann, Bodo Brueckner and Frank Lyko
volume 6 | issue 9
2 May 2007Pages: 1001 - 1005
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MicroRNAs (miRNAs) are small RNA molecules that control gene expression by inhibition of protein translation or by degradation of cognate target mRNAs. Even though strict developmental and tissue-specific regulation appears to be critical for miRNA function, very little is known about the mechanisms governing miRNA gene expression. Several recent studies have shown that miRNA genes can be regulated by DNA methylation and other epigenetic mechanisms. The observation of altered miRNA gene methylation patterns in human cancers also suggested that miRNA gene methylation is functionally relevant for tumorigenesis. We have now performed a comprehensive analysis of miRNA genes and found that about half of these genes are associated with CpG islands and thus represent candidate targets of the DNA methylation machinery. An expanded analysis of several miRNA-associated CpG islands in five cell lines indicated that miRNA gene methylation is detectable at high frequencies, both in normal and malignant cells. Possible explanations for this phenomenon include the specific structure of miRNA genes and/or their requirement for strict expression regulation.
Authors
Barbara Weber
Deutsches Krebsforschungszentrum, Heidelberg, Germany
Carlo Stresemann
Deutsches Krebsforschungszentrum, Heidelberg, Germany
Bodo Brueckner
Deutsches Krebsforschungszentrum, Heidelberg, Germany
Frank Lyko
Deutsches Krebsforschungszentrum, Heidelberg, Germany
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.