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Methodological Report
Interspecies Comparative Genomic Hybridization (I-CGH): A New Twist to Study Animal Tumor Models
Sivakumar Jaikumar, Zhengping Zhuang, Poonam Mannan, Alexander O. Vortmeyer, Makoto Furuta, Rob Dickerman, Julia Bedanova, Russell R. Lonser, Stuart Walbridge, Robert J. Weil, Victor V. Lobanenkov, Edward H. Oldfield and Svetlana D. Pack
volume 6 | issue 7
1 April 2007Pages: 836 - 842
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Animal models of human diseases are widely used to address questions of tumor development. Selection of a particular animal model depends upon a variety of factors, among them: animal cost, species lifespan, and hardiness; availability of biomolecular and genetic tools for that species; and evolutionary distance from humans. In spite of the growth in genomic data in the past several years, many animal models cannot yet be studied extensively due to gaps in genetic mapping, sequencing and functional analyses. Thus, alternative molecular genetic approaches are needed. We have designed an interspecies comparative genomic hybridization approach to analyze genetic changes in radiation-induced brain tumors in the non-human primate, Macaca mulatta. Using homologies between the primate and human genomes, we adapted widely-available CGH techniques to generate cytogenetic profiles of malignant gliomas in 4 monkey tumors. Losses and gains were projected onto the corresponding homologous chromosomal regions in the human genome, thus directly translating the status of the monkey gliomas into human gene content. This represents a novel method of comparative interspecies cytogenetic mapping that permits simultaneous analysis of genomic imbalance of unknown sequences in disparate species and correlation with potential or known human disease-related genes.
Authors
Sivakumar Jaikumar
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland
Zhengping Zhuang
National Institutes of Health, Bethesda, MD
Poonam Mannan
National Institutes of Health, Bethesda, MD
Alexander O. Vortmeyer
National Institutes of Health, Bethesda, MD
Makoto Furuta
National Institutes of Health, Bethesda, MD
Rob Dickerman
National Institutes of Health, Bethesda, MD
Julia Bedanova
National Institutes of Health, Bethesda, MD
Russell R. Lonser
National Institutes of Health, Bethesda, MD
Stuart Walbridge
National Institutes of Health, Bethesda, MD
Robert J. Weil
Cleveland Clinic Foundation, Cleveland, Ohio
Victor V. Lobanenkov
National Institutes of Health, Rockville, MD
Edward H. Oldfield
National Institutes of Health, Bethesda, MD
Svetlana D. Pack
National Institutes of Health, Bethesda, MD
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.