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Brief Report
Oxidative Stress Overrides the Spindle Checkpoint
Vincenzo D'Angiolella, Concetta Santarpia and Domenico Grieco
volume 6 | issue 5
1 March 2007Pages: 576 - 579
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Aneuploidy, an abnormal chromosome set, can ensue from failure of the spindle checkpoint, the safeguard mechanism that halts anaphase onset until mitotic spindle assembly. Inefficiency of cells to maintain the normal chromosome set across cell generations has been linked to tumorigenesis and senescence. Here we show that oxidative stress overrides the spindle checkpoint mechanism. Oxidant challenge of checkpoint-arrested cells led to proteolysis of the anaphase inhibitor securin and mitotic cyclins. This appeared consequent to loss of cyclin B-cdk1 activity caused by oxidant-induced reversal of cdk1 inhibitory phosphorylation. These observations may provide a link between aneuploidy occurrence and oxidative stress.
Authors
Vincenzo D'Angiolella
University of Napoli “Federico II”, Napoli, Italy
Concetta Santarpia
University of Napoli “Federico II”, Napoli, Italy
Domenico Grieco
University of Napoli “Federico II”, Napoli, Italy
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.