CTCF Regulates Asynchronous Replication of the Imprinted H19/Igf2 Domain

Rosita Bergström, Joanne Whitehead, Sreenivasulu Kurukuti and Rolf Ohlsson

volume 6 | issue 4

15 February 2007
Pages: 450 - 454

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Asynchronous replication during S phase is a universal characteristic of genomically imprinted genes. Replication timing in imprinted domains is determined epigenetically, as it is parent of origin specific, and is seen in the absence of sequence divergence between the two alleles. At the imprinted H19/Igf2 domain, the methylated paternal allele replicates early while the CTCF-bound maternal allele replicates late during S phase. CTCF regulates the allele-specific epigenetic characteristics of this domain, including methylation, transcription and chromosome conformation. Here we show that maternal, but not paternal inheritance of a mutated H19 imprinting control region, lacking functional CTCF binding sites, underlies a late to early switch in replication timing of the maternal H19/Igf2 domain.

Authors

Rosita Bergström

Uppsala University, Uppsala, Sweden

Joanne Whitehead

CNRS & Institut Curie, Paris, France

Sreenivasulu Kurukuti

The Babraham Institute, Cambridge, UK

Rolf Ohlsson