Pim-1 Phosphorylates the DNA Binding Domain of c-Myb
Volume 2, Issue 3
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Pages 257 - 261http://dx.doi.org/10.4161/cc.2.3.383
Authors: Louise M. Winn, Wanli Lei and Scott A. Ness View affiliations
The c-Myb transcription factor regulates cellular differentiation and proliferation and is regulated by complex mechanisms that control its repressed oncogenic activity. The transcriptional activity of c-Myb is regulated by the serine/threonine protein kinase Pim-1. Here, we show that Pim-1 is able to interact with c-Myb and the closely related transcription factor A-Myb, via direct interactions with the highly conserved Myb DNA binding domain. Pim-1 associated with Myb both in cells and in vitro, and phosphorylated the Myb DNA binding domain, suggesting that it regulates Myb protein activity by direct phosphorylation.