Sign up for Table of Contents Alerts.
Email this page
Print this page
Report
CYFIP2, a Direct p53 Target, is Leptomycin-B Sensitive
Roger S. Jackson II, Yong-Jig Cho, Susanne Stein and Peng Liang
volume 6 | issue 1
1 January 2007Pages: 95 - 103
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
A number of target genes for the tumor suppressor, p53, have been identified, however, the mechanisms that contribute to p53-dependent apoptosis remain to be fully elucidated. In a comprehensive screen for p53 target genes, we have identified Cytoplasmic FMR Interacting Protein 2 (CYFIP2) as a p53-inducible gene. Here we show that the CYFIP2 promoter contains a p53-responsive element that confers p53 binding as well as transcriptional activation of a heterologous reporter. Inducible expression of CYFIP2 is sufficient for caspase activation and cellular apoptosis, reminiscent of p53 activation. Together, these results suggest that CYFIP2 is a direct p53 target gene that may be part of a redundant network of genes responsible for p53-dependent apoptosis. In addition, the sensitivity of CYFIP2 protein subcellular localization to Leptomycin-B, a Crm-1/Exportin inhibitor, suggests that the biological functions of CYFIP2 may extend from the cytoplasmic compartment into the nucleus of the cell.
Authors
Roger S. Jackson II
Vanderbilt University Medical Center, Nashville, TN
Yong-Jig Cho
Vanderbilt University Medical Center, Nashville, TN
Susanne Stein
Vanderbilt University Medical Center, Nashville, TN
Peng Liang
Vanderbilt University Medical Center, Nashville, TN
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.