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Reports

Identification of New p63 Targets in Human Keratinocytes

Barbara Testoni, Serena Borrelli, Elena Tenedini, Daniela Alotto, Carlotta Castagnoli, Stefano Piccolo, Enrico Tagliafico, Sergio Ferrari, M Alessandra Viganò and Roberto Mantovani

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p63 is a transcription factor involved in the development of ectodermal tissues, including limb, skin and, in general, multilayered epithelia. We identified both activated and repressed genes in human keratinocytes via gene expression profiling of p63-depleted cells and validated 21 new primary targets by RT-PCR and ChIP location analysis. The p63 isoforms differentially activate or repress selected promoters. ChIPs in primary keratinocytes indicate that p63 targets are generally shared with p53, but some are p63-specific. Several growth suppressors are among repressed genes. The newly identified genes belong to pathways of growth and differentiation and are regulated in HaCaT differentiation and in stratification of human skin.

Authors

Barbara Testoni

Dipartimento di Scienze Biomolecolari e Biotecnologie, Milano, Italy.

Serena Borrelli

Dipartimento di Scienze Biomolecolari e Biotecnologie, Milano, Italy

Elena Tenedini

Dipartimento di Scienze Biomediche, Modena, Italy

Daniela Alotto

Dipartimento di Chirurgia Plastica, Torino, Italy.

Carlotta Castagnoli

Dipartimento di Chirurgia Plastica, Torino, Italy

Stefano Piccolo

Dipartimento di Istologia, Microbiologia e Biotecnologie Mediche, Università di Padova, Italy

Enrico Tagliafico

Dipartimento di Scienze Biomediche, Modena, Italy

Sergio Ferrari

Dipartimento di Scienze Biomediche, Modena, Italy

M Alessandra Viganò

Dipartimento di Scienze Biomolecolari e Biotecnologie, Milano, Italy

Roberto Mantovani

Dipartimento di Scienze Biomolecolari e Biotecnologie, Milano, Italy

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.