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Cell Cycle Regulatory Proteins in the Liver in Murine Trypanosoma cruzi Infection
Boumediene Bouzahzah, Fnu Nagajyothi, Mohan Krishnamachary, Alex Cohen, Michael P. Lisanti, Murry Wittner, Shankar Mukherjee and Chris Albanese
volume 5 | issue 20
15 october 2006Pages: 2396 - 2400
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The liver is an important target of Trypanosoma cruzi infection. Infection of CD-1 mice with T. cruzi (Brazil strain) resulted in parasitism of the liver, primarily in sinusoidal and Kupffer cells. Immunoblot analysis revealed activation of extra cellular signal-regulated kinase (ERK) during the acute and subacute period of infection, but p38 mitogen activated kinase (MAPK) and JNK were not activated. The activity of important cell cycle regulatory genes was also examined in the liver following infection. There was increased expression of cyclin D1, cyclin E and cyclin A as well as proliferating cell nuclear antigen (PCNA) at 45, 60 and 215 days post infection. In addition, the expression of the cyclin-dependent kinase inhibitors p27KIP1, p21WAF1 and the tumor suppressor p53 were increased in the liver obtained from infected mice. Quantitative PCR revealed increased abundance of mRNA for cyclins A, D1 and E. Interestingly, cyclin A and E are ordinarily not found in the adult liver. Thus infection caused a reversion to a fetal/neonatal phenotype. These data provide a molecular basis for cell proliferation in the liver following T. cruzi infection.
Authors
Boumediene Bouzahzah
Albert Einstein College of Medicine
Fnu Nagajyothi
Albert Einstein College of Medicine; Bronx, NY
Mohan Krishnamachary
Albert Einstein College of Medicine
Alex Cohen
Albert Einstein College of Medicine, Bronx, New York
Michael P. Lisanti
Thomas Jefferson University; Philadelphia, PA
Murry Wittner
Albert Einstein College of Medicine, Bronx, New York
Shankar Mukherjee
Albert Einstein College of Medicine, Bronx, New York
Chris Albanese
Georgetown University Medical Center; Washington DC USA
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.