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Condensin Function in Mitotic Nucleolar Segregation is Regulated by rDNA Transcription

Bi-Dar Wang, Pavel Butylin and Alexander Strunnikov

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Chromosome condensation is established and maintained by the condensin complex. The mechanisms governing loading of condensin onto specific chromosomal sites remain unknown. To elucidate the molecular pathways that determine condensin binding to the nucleolar organizer, a key condensin binding site, we analyzed the properties of condensin-bound sites within the rDNA repeat in budding yeast and demonstrated that the bulk of mitotic condensin binding to rDNA is reduced or eliminated when Pol I transcription is elevated. Conversely, when Pol I transcription is repressed either by rapamycin treatment or by promoter shut-off, condensin binding to rDNA is increased. This novel potential role for constitutive and/or periodic repression of Pol I transcription in rDNA condensin loading is an important factor in determining the segregation proficiency of NOR-containing chromosomes.

Authors

Bi-Dar Wang

NICHD, Laboratory of Gene Regulation and Development, Bethesda MD USA

Pavel Butylin

NICHD, Laboratory of Gene Regulation and Development, Bethesda MD USA

Alexander Strunnikov

NICHD, Laboratory of Gene Regulation and Development, Bethesda MD USA

This is an open-access article

 Download PDF

If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.