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A High-Throughput Loss-of-Function Screening Identifies Novel p53 Regulators
Susana Llanos, Alejo Efeyan, Jorge Monsech, Orlando Dominguez and Manuel Serrano
volume 5 | issue 16
15 august 2006Pages: 1880 - 1885
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The p53 protein is a sequence-specific transcription factor that plays a crucial role in tumor suppression by inducing apoptosis or cell cycle arrest in response to cellular damage. To identify novel proteins involved in the regulation of p53 transcriptional activity we have performed a large scale RNA interference-based screen. We have identified four genes previously unknown to be involved in modulating p53 activity (GAS41, RPS6K4, RUNDC1, and CRMP-2). The interference of each of these four genes resulted in the up-regulation of p53 transcriptional activity and, conversely, their over-expression resulted in the inhibition of p53 target promoters and p53-mediated apoptosis. These observations suggest a role for these genes as p53 inhibitors and imply that they may have oncogenic activity.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.