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A Novel Guanine Nucleotide Exchange Factor, MYOGEF, is Required for Cytokinesis
Di Wu, Michael Asiedu, Robert S. Adelstein and Qize Wei
volume 5 | issue 11
1 june 2006Pages: 1234 - 1239
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The cleavage furrow is created by an actomyosin contractile ring that is regulated by small GTPase proteins such as Rac1 and RhoA. Guanine nucleotide exchange factors (GEFs) are positive regulators of the small GTPase proteins and have been implicated as important factors in regulating cytokinesis. However, it is still unclear how GEFs regulate the contractile ring during cytokinesis in mammalian cells. Here we report that a novel GEF, which is termed MyoGEF (myosin-interacting GEF), interacts with nonmuscle myosin II and exhibits activity toward RhoA. MyoGEF and nonmuscle myosin II colocalize to the cleavage furrow in early anaphase cells. Disruption of MyoGEF expression in U2OS cells by RNA interference (RNAi) results in the formation of multinucleated cells. These results suggest that MyoGEF, RhoA, and nonmuscle myosin II act as a functional unit at the cleavage furrow to advance furrow ingression during cytokinesis.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.