Sign up for Table of Contents Alerts.
Email this page
Print this page
Report
Protein Kinase A(PKA)-Restrictcive and PKA-Permissive Phases of Oocyte Maturation
Jing Wang, Winnie Li Cao and X. Johné Liu
volume 5 | issue 2
16 january 2006Pages: 213 - 217
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
Substantial evidence has indicated that cAMP-dependent protein kinase (protein kinase A or PKA) plays a critical role in maintaining meiotic prophase arrest in vertebrate oocytes. However, PKA activity dynamic and its physiological substrate profile remain poorly defined. We have recently developed a novel PKA substrate construct which we employ to monitor PKA activity in live oocytes. In the current study, we have employed biochemical and imaging analyses of single cells to determine PKA activity dynamics during oocyte maturation and to investigate the consequence of re-activation of PKA during oocyte maturation. We demonstrated here that progesterone caused a rapid and permanent inhibition of PKA during the entire maturation process. However, artificial reactivation of endogenous PKA had differential consequences, depending on the timing of PKA reactivation. Reactivation of endogenous PKA at any time prior to GVBD inhibited progesterone-induced GVBD. PKA reactivation at GVBD, or thereafter, did not interfere with meiosis I to meiosis II transition, nor did it interfere with metaphase II arrest. These results demonstrate for the first time a PKA-restricted phase and a PKA-permissive phase during oocyte maturation.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.