Septin 7 is required for orderly meiosis in mouse oocytes
Volume 11, Issue 17
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September 1, 2012
Pages 3211 - 3218http://dx.doi.org/10.4161/cc.21553
: Septin 7, meiosis, mouse, oocyte, overexpression
Authors: Sen Li, Xiang-Hong Ou, Liang Wei, Zhen-Bo Wang, Qing-Hua Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten and Qing-Yuan Sun View affiliations
Septin 7 is a conserved GTP-binding protein. In this study, we examined the localization and functions of Septin 7 during mouse oocyte meiotic maturation. Immunofluorescent analysis showed that intrinsic Septin 7 localized to the spindles from the pro-MI stage to the MII stage. Knockdown of Septin 7 by siRNA microinjection caused abnormal spindles and affected extrusion of the first polar body. Septin 7 mRNA tagged with myc was injected into GV stage oocytes to overexpress Septin 7. Overexpressed Myc-Septin 7 localized to the spindle and beneath the plasma membrane displaying long filaments. Fluorescence intensity of spindle α-tubulin in myc-Septin 7-injected oocytes was weaker than that of the control group, demonstrating that Septin 7 may influence recruitment of α-tubulin to spindles. MII oocytes injected with myc-Septin 7 exhibited abnormal chromosome alignment, and parthenogenetic activation failed to allow extrusion of the second polar body, suggesting that overexpression of Septin 7 may affect extrusion of the polar body by disturbing the alignment of chromosomes and regulating α-tubulin recruitment to spindles. In summary, Septin 7 may regulate meiotic cell cycle progression by affecting microtubule cytoskeletal dynamics in mouse oocytes.
Received: June 11, 2012; Accepted: July 19, 2012; Published Online: August 16, 2012
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