Report

pH neutralization protects against reduction in replicative lifespan following chronological aging in yeast

Volume 11, Issue 16   August 15, 2012
Pages 3087 - 3096
http://dx.doi.org/10.4161/cc.21465
Keywords: chronological lifespan, longevity, replication stress, replicative lifespan, yeast
Authors: Christopher Murakami, Joe R. Delaney, Annie Chou, Daniel Carr, Jennifer Schleit, George L. Sutphin, Elroy H. An, Anthony S. Castanza, Marissa Fletcher, Sarani Goswami, Sean Higgins, Mollie Holmberg, Jessica Hui, Monika Jelic, Ki-Soo Jeong, Jin R. Kim, Shannon Klum, Eric Liao, Michael S. Lin, Winston Lo, Hillary Miller, Richard Moller, Zhao J. Peng, Tom Pollard, Prarthana Pradeep, Dillon Pruett, Dilreet Rai, Vanessa Ros, Alex Schuster, Minnie Singh, Benjamin L. Spector, Helen Vander Wende, Adrienne M. Wang, Brian M. Wasko, Brady Olsen and Matt Kaeberlein

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Abstract:
Chronological and replicative aging have been studied in yeast as alternative paradigms for post-mitotic and mitotic aging, respectively. It has been known for more than a decade that cells of the S288C background aged chronologically in rich medium have reduced replicative lifespan relative to chronologically young cells. Here we report replication of this observation in the diploid BY4743 strain background. We further show that the reduction in replicative lifespan from chronological aging is accelerated when cells are chronologically aged under standard conditions in synthetic complete medium rather than rich medium. The loss of replicative potential with chronological age is attenuated by buffering the pH of the chronological aging medium to 6.0, an intervention that we have previously shown can extend chronological lifespan. These data demonstrate that extracellular acidification of the culture medium can cause intracellular damage in the chronologically aging population that is asymmetrically segregated by the mother cell to limit subsequent replicative lifespan.

Received: July 11, 2012; Accepted: July 12, 2012; Published Online: August 8, 2012

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