Special Report

Evidence That the Yeast Spindle Assembly Checkpoint Has a Target Other Than the Anaphase Promoting Complex

Volume 4, Issue 11   November 2005
Pages 1555 - 1557
http://dx.doi.org/10.4161/cc.4.11.2144
Authors: Catherine A. Andrews, Laura A. Díaz-Martínez, Juan F. Gimenez-Abian and Duncan J. Clarke

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Abstract:
The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.

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