Report
Analysis of centrosome localization of BRCA1 and its activity in suppressing centrosomal aster formation
Purchase or Subscribe
Pages 2931 - 2946
http://dx.doi.org/10.4161/cc.21396
Keywords: BRCA1, aster formation, centriole, centrosome, microtubules
Authors: Pheruza Tarapore, Kazuhiko Hanashiro and Kenji Fukasawa
View affiliations
Abstract:
BRCA1, a product of a familial breast and ovarian cancer susceptibility gene, localizes to centrosomes and physically interacts with γ-tubulin, a key centrosomal protein for microtubule nucleation and anchoring at centrosomes. Here, we performed a rigorous analysis of centrosome localization of BRCA1, and found that BRCA1 is specifically associated with mother centrioles in unduplicated centrosomes, and daughter centrioles acquire BRCA1 prior to initiation of duplication, and thus duplicated centrosomes are both bound by BRCA1. We further found that BRCA1 suppresses centrosomal aster formation. In addition, we identified a new domain of BRCA1 critical for γ-tubulin binding, which confers not only its localization to centrosomes, but also its activity to suppress centrosomal aster formation.
Received: April 3, 2012; Accepted: July 6, 2012
Preview:
Full Text - Ahead of Print Available - Log in!
