Report
Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe
Downloads and Tools
Article PDF
1.3 MB PDF document
XML File
Full Text XML
78.55 KB XML Document
Supplemental Material
- Supplemental File
1.05 MB PDF document
This is an open access article.
Print this page
Email this page
Article Citation
RIS MARC (XML) FULL CITATION
Share on Facebook
Pages 3527 - 3532
http://dx.doi.org/10.4161/cc.10.20.17792
Keywords: analog-sensitive, conditional allele, fission yeast, kinase, phosphorylation
Authors: Lubos Cipak, Chao Zhang, Ines Kovacikova, Cornelia Rumpf, Eva Miadokova, Kevan M. Shokat and Juraj Gregan
View affiliations
Abstract:
The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein kinases that are essential for viability. Studies of the essential kinases often require the use of mutant strains carrying conditional alleles. To inactivate these kinases conditionally, we applied a recently developed chemical genetic strategy. The mutation of a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors, allowing for specific inactivation of the modified kinase. Using this approach, we constructed conditional analog-sensitive alleles of 13 essential protein kinases in the fission yeast S. pombe.
Received: July 26, 2011; Accepted: August 17, 2011
Preview:
Advertisements
