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Research Paper
Differentially Expressed Apoptotic Genes in Early Stage Lung Adenocarcinoma Predicted by Expression Profiling
Sunil Singhal, Kunjlata M. Amin, Robert Kruklitis, M. Blair Marshall, John C. Kucharczuk, Peter DeLong, Leslie A. Litzky, Larry R. Kaiser and Steven M. Albelda
volume 2 | issue 5
sept/oct 2003Pages: 566-571
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OBJECTIVE: In undiseased lung epithelial cells, apoptosis is an evolutionarily conserved and genetically regulated form of cell suicide which plays an important role in development and in the maintenance of tissue homeostasis. Neoplastic lung cells develop the ability to deregulate growth by alterations in these genes which control apoptosis. Genomic profiling was used to compare gene expression levels in early stage lung adenocarcinomas and non-neoplastic pulmonary tissue in order to comprehensively identify alterations in the process of apoptosis.
METHODS: RNA extracted from node negative, poorly differentiated lung adenocarcinomas (15 patients) and non-neoplastic pulmonary tissue (5 patients) was hybridized to oligonucleotide microarray filters containing 44,363 genes. Ontological classification was used to extract genes involved with apoptosis. Further analysis discovered a subset of differentially expressed genes for further study.
RESULTS: Of the 308 apoptotic genes on the microarray filters, 24 genes were predicted to be differentially expressed in lung adenocarcinomas. Alterations in several genes (ie. Akt, BcL-xL, PTEN, FAS) are consistent with the literature. We also identified 10 novel genes that have not been described in non-small cell lung cancer (ie. RIP, Caspase 1, PDK-1).
CONCLUSIONS: These results identified several potential apoptotic genes altered in lung cancer.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.