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Research Paper
Effect of Dietary Tetradecylthioacetic Acid on Colon Cancer Growth Studied by Dynamic Contrast Enhanced MRI
Line R. Jensen, Kjetil Berge, Tone F. Bathen, Hege Wergedahl, Svanhild A. Schønberg, Anna Bofin, Rolf K. Berge and Ingrid S. Gribbestad
volume 6 | issue 11
November 2007Pages: 1810 - 1816
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Purpose: To evaluate the effects of diets supplemented with the modified fatty acid tetradecylthioacetic acid (TTA) and fish protein hydrolysate (FPH) on tumor growth of the human colon cancer cell line SW620, and to investigate the properties of tumor vasculature by dynamic contrast-enhanced MRI in a human tumor xenograft. Experimental design: SW620 cells were grown in vitro in presence of TTA and palmitic acid and proliferation was measured by thymidine incorporation. The xenograft study in mice was performed with four distinct diets: (a) control diet; (b) diet with TTA; (c) diet with TTA and FPH; and (d) diet with FPH. SW620 cells were injected subcutaneously, and Dynamic Contrast Enhanced (DCE) MRI was performed on a Bruker BioSpec 7T system. The data was analyzed by two-compartment modeling of the contrast enhancement, initial area under the curve (IAUC) and by use of relative signal intensity (RSI) distributions. Results: The in vitro cell studies revealed that TTA reduced tumor cell proliferation as a function of both dose and time. The in vivo tumor growth was significantly reduced for the two groups fed TTA, as compared to the control group. The mean 10th percentile RSI, ve and IAUC for the TTA group were significant higher than for the control group Conclusions: This study confirms the growth inhibitory effects of TTA, both in vitro and in vivo, in a colon cancer model. The analysis of DCE MRI data showed that TTA influences the vascular properties of the tumor in addition to the growth.
Authors
Line R. Jensen
Norwegian University of Science and Technology (NTNU), Trondheim, Norway
Kjetil Berge
University of Bergen, Bergen, Norway
Tone F. Bathen
Norwegian University of Science and Technology (NTNU), Trondheim, Norway
Hege Wergedahl
University of Bergen, Bergen, Norway
Svanhild A. Schønberg
Norwegian University of Science and Technology, Trondheim, Norway
Anna Bofin
Norwegian University of Science and Technology, Trondheim, Norway
Rolf K. Berge
University of Bergen, Bergen, Norway
Ingrid S. Gribbestad
Norwegian University of Science and Technology (NTNU), Trondheim, Norway
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.