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Research Paper
Functional Expression of TLR9 is Associated to the Metastatic Potential of Human Lung Cancer Cell
Tao Ren, Zhen-Ke Wen, Zhong-Min Liu, Yong-Jie Liang, Zhong-Liang Guo and Lin Xu
volume 6 | issue 11
November 2007Pages: 1704 - 1709
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CpG-oligonucleotides (CpG-ODN), which induce signaling through Toll-like receptor 9 (TLR9), are widely used as adjuvants in therapy against cancer. However, tumor cells express functional TLR9 were recently reported and the immune effect of CpG ODN on tumor cells remains unclear. Here we investigated the direct effects of CpG ODN on human tumor cell line 95D cells using flow cytometric analysis and western blotting. We found strongly high expression of TLR9 in 95D cells. Stimulation of 95D cells with CpG-ODN induced significantly elevated secretion of IL-1α and IL-8, as well as the expression of CXCR4, ICAM-1 and MMP-2. Furthermore, the invasion of 95D cells and TLR9 modifying 95C cells were significantly enhanced by stimulation of CpG ODN, which could be abrogated by inhibitory CpG ODN and chloroquine. These results suggest that functionally active TLR9 is expressed on human tumor cell lines, and may represent a novel insight on the role of TIL9 agonist used in tumor immunotherapy.
Authors
Tao Ren
Department of Respiratory Medicine, East Hospital, Tongji University, Shanghai, China
Zhen-Ke Wen
Department of Central Laboratory, Qingdao Blood Center, Shandong, China
Zhong-Min Liu
Department of Cardiothoracic Surgery, East Hospital, Tongji University, Shanghai, China
Yong-Jie Liang
East Hospital, Tongji University, Shanghai, China
Zhong-Liang Guo
East Hospital, Tongji University, Shanghai, China
Lin Xu
Department of Immunology, Zunyi Medical College, Guizhou, China
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.