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Research Paper
Hela l-CaD Undergoes a DNA Replication-Associated Switch in Localization From the Cytoplasm to the Nuclei of Endothelial Cells/Endothelial Progenitor Cells in Human Tumor Vasculature
Ping-Pin Zheng, Marcel van der Weiden, Peter A.E. Sillevis Smitt, Theo M. Luider and Johan M. Kros
volume 6 | issue 6
June 2007We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
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Caldesmon (CaD) is a major actin-binding protein distributed in a variety of cell types. So far no diversity in functions of the different isoforms were found in in vitro studies. The low molecular weight isoform (Hela l-CaD) was detected in the vasculature of a variety of tumor types in our previous study. Proliferation of endothelial cells/endothelial progenitor cells is a crucial event for formation of new blood vessels. Here we report the intranuclear translocation of Hela l-CaD in cell cycle activated ECs/EPCs in the vasculature of human tumors. The nuclear translocation coincides with phosphorylation of the molecule and the activation of intranuclear protein kinase p34cdc2. These findings point to a function of this molecule relating to DNA synthesis which is triggered by cell-cycle signalling pathways. The data challenge and update the generally accepted concept that CaD is a pure cytoplasmic protein in vitro study. It suggests that nuclear translocation of Hela l-CaD serves as an additional regulatory step in the control of mitotic initiation and triggers further investigations in the role of this protein in the regulation of nuclear functions.
Authors
Ping-Pin Zheng
Department of Pathology, Erasmus Medical Center, Rotterdam, The Netherlands
Marcel van der Weiden
Department of Pathology, Erasmus Medical Center, Rotterdam, The Netherlands
Peter A.E. Sillevis Smitt
Erasmus Medical Centre, Rotterdam, The Netherlands
Theo M. Luider
Erasmus Medical Centre, Rotterdam, The Netherlands
Johan M. Kros
Department of Pathology, Erasmus Medical Center, Rotterdam, The Netherlands
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.