Sign up for Table of Contents Alerts.
Email this page
Print this page
Research Paper
Zebrafish tp53 Knockdown Extends the Survival of Irradiated Zebrafish Embryos More Effectively than the p53 Inhibitor Pifithrin
Kevin T. Duffy and Eric Wickstrom
volume 6 | issue 5
May 2007Pages: 675 - 678
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
Cancer treatment strategies using radiation therapy have significantly improved outcomes in patients. Although advances in physics and electronics have enabled physicians to plan and deliver radiotherapy regimens precisely and efficiently, studies of the underlying molecular mechanisms of the radiation response could enable more effective and less toxic treatment strategies, as well as protection against radiation damage following unplanned exposure. Using the developing zebrafish embryo as a model system, we hypothesized that knockdown of tp53 mRNA or small molecule inhibition of p53 could alter the efficacy of radiotherapy against developing zebrafish embryos as a model for tumors. We found that interfering with p53 expression and function using hydroxylprolyl-phosphono peptide nucleic acid oligomers or pifithrin-α protected developing zebrafish embryos against radiation damage, consistent with our hypothesis.
Authors
Kevin T. Duffy
Thomas Jefferson University, Philadelphia, PA
Eric Wickstrom
Thomas Jefferson University, Philadelphia, PA
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.