Palladin is overexpressed in the non-neoplastic stroma of infiltrating ductal adenocarcinomas of the pancreas, but is only rarely overexpressed in neoplastic cells
Volume 6, Issue 3
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Pages 324 - 328http://dx.doi.org/10.4161/cbt.6.3.3904
Authors: Safia N. Salaria, Peter Illei, Rajni Sharma, Kimberly M. Walter, Alison Klein, James R. Eshleman, Anirban Maitra, Richard D. Schulick, Jordan M. Winter, Michel M. Ouellette, Michael Goggins and Ralph Hruban View affiliations
Background: It has recently been suggested that over expression of palladin in sporadic pancreatic cancer may contribute to pancreatic cancer’s invasive and migratory abilities. This hypothesis was based on reverse transcriptase-polymerase chain reaction analyses of bulk pancreatic tissue, yet pancreatic cancer is a complex admixture of neoplastic epithelial cells and desmoplastic stroma.
Design: Immunohistochemical labeling of tissue microarrays was used to define the patterns of palladin protein expression in 177 ductal adenocarcinomas of the pancreas. Western blot analysis was used to determine the epitope(s) of palladin recognized by the antibody as well as the relative levels of palladin expression in short-term cultures of stromal fibroblasts, non-neoplastic ductal cells and pancreatic cancer cell lines.
Results: Immunolabeling revealed that the palladin protein was strongly over expressed in non-neoplastic stromal cells in 171 (96.6%) of the 177 evaluable pancreatic cancers. By contrast, the over expression of palladin protein by the neoplastic epithelial cells relative to normal pancreatic epithelium was observed in only 22 (12.4%) of the 177 cancers. Western blot analysis confirmed that the antibody recognizes the ~90 kDa isoform of palladin, and demonstrated that fibroblast cell lines had higher expression of palladin than pancreatic cancer cell lines.
Conclusions: The over expression of palladin relative to normal pancreas in the majority of pancreatic cancers is limited to non-neoplastic stromal cells. This observation highlights the limitations of relying on bulk tissues when analyzing gene expression. Since palladin is not over expressed in most pancreatic cancer cells, the over expression of palladin is not likely to be responsible for pancreatic cancer cells invasive and migratory abilities.