Sign up for Table of Contents Alerts.
Email this page
Print this page
Research Paper
Visual Genotyping of a Coat Color Tagged p53 Mutant Mouse Line
Binhai Zheng, Hannes Vogel, Lawrence A. Donehower, and Allan Bradley
volume 1 | issue 4
July/August 2002Pages: 433-435
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
The p53 knockout mouse has been widely used as a model in cancer research and other applications. Because neither homozygous nor heterozygous mutant p53 mice exhibit an overt phenotype, each animal requires laborious molecular genotyping. Here we describe a new p53 mutant mouse that is tagged with a tyrosinase coat color minigene. On an albino background, heterozygous tyrosinase-tagged p53 mutant mice exhibit a light tan coat color, while homozygous mutants display a darker brown coat color. Thus, by 8-10 days of age, mice with two, one, or no mutant p53 alleles are immediately distinguishable by their coat color, eliminating the time, costs, and errors associated with molecular genotyping. Moreover, the homozygous mutant p53-tyrosinase mice display a tumor incidence and spectrum virtually identical to previous p53 null mouse lines. Thus, tagging targeted mutations with such coat color markers provides a generally applicable genotyping method for embryonic stem cell-derived mice.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.