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Research Paper
Adenovirus-Mediated Ink4a/ARF Gene Transfer Significantly Suppressed the Growth of Pancreatic Carcinoma Cells
Fang Chen, Yiting Li, Zhaohui Lu, Jie Gao and Jie Chen
volume 4 | issue 12
december 2005Pages: 1348-1354
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Objectives: To determine the effects of adenovirus-mediated transfer of p14ARF and p16INK4a on growth and apoptosis of human pancreatic carcinoma cell lines. Results: Pancreatic carcinoma cell lines, PC-7, PANC-1 and MIA PaCa-2 (p14ARF and p16INK4a -/-), were used. PC-7 (p53 wt) and MIA PaCa-2 (p53 mt) cells infected with recombinant adenovirus vector expressing p14ARF gene (Adp14) showed significant inhibition of cell growth compared with control vector in vitro and in vivo, whereas PANC-1 cells (p53 mt) did not show such effects. Those three cell lines exhibited growth retardation and senescence phenotype after infected with the adenovirus vector containing p16INK4a gene (Adp16) in vivo and in vitro. Conclusions: Adenovirus-mediated transfer of p14ARF and p16INK4a produces significant growth suppression of pancreatic carcinoma cells in vitro and in vivo. The effects of Adp14 partly depend on the status of p53 gene in those cells.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.