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Research Paper
Heterogeneity in Non-Invasive Detection of Apoptosis Among Human Tumor Cell Lines Using Annexin-V Tagged with EGFP or Qdot-705
David T. Dicker, Seok-Hyun Kim, Zhaoyu Jin and Wafik S. El-Deiry
volume 4 | issue 9
september 2005Pages: 1014-1017
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The ability to measure apoptosis in vivo and in vitro provides an invaluable tool to assess the effects of anti-cancer agents. The annexin-V binding assay has been used extensively, including in the clinic as a Technetium conjugate to assess response to chemo- or radio-therapy. Our results reveal that not all tumor cell lines are amenable to this assay. We investigated ten different human cancer cell lines for their staining patterns with annexin-V during apoptosis. In six human tumor cell lines (HCT116, Jurkat, FADU, PA1, SkBr3, and Saos2) annexin-V staining correlated well with another measurement of apoptosis (APOSTAIN). Four cell lines (SW480, H460, MCF7, and DLD1) showed annexin-V staining which could not be interpreted, where APOSTAIN demonstrated a clear apoptotic population. The poor annexin-V staining of some tumor cells undergoing apoptosis was not correlated with detachment procedures or choice of apoptosis-inducing agent. Use of annexin-V tagged with EGFP or Qdot-705 can reliably detect apoptosis in some but not all human tumors undergoing apoptosis in response to chemotherapy or TRAIL.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.