Sign up for Table of Contents Alerts.
Email this page
Print this page
Research Paper
Analysis of the Mononuclear Inflammatory Cell Infiltrate in the Nontumorigenic, Pretumorigenic and Tumorigenic Keratinocytic Hyperproliferative Lesions of the Skin
Mahmoud R. Hussein and Rabab A. Ahmed
volume 4 | issue 8
august 2005Pages: 819-821
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.
BACKGROUND: The keratinocytic hyperproliferative lesions include non-tumorigenic, pretumorigenic (actinic keratoses, AK), and tumorigenic (squamous cell carcinomas, SCC) conditions. Although mononuclear inflammatory cell infiltrate (MICs) is a constant feature in these lesions, their immunophenotypic characterization is still incomplete. We hypothesized that the development of non-tumorigenic, pretumorigenic, and tumorigenic keratinocytic hyperproliferative lesions is associated with alterations in the mononuclear inflammatory cell infiltrate in response to altered antigenicity of the lesional cells. This study tries to test this hypothesis and to characterize MICs in these lesions. METHODS: Fifty lesions (nontumorigenic lesions, 29; AK, 9 and SCC, 12) were examined using immunoperoxidase staining methods and antibodies targeting histiocytes (CD68), T (CD3), B cells (CD20), and T cells with cytotoxic potential (TIA-1). RESULTS: As compared to the normal skin, the development of the keratinocytic hyperproliferative lesions (normal skin; non-tumorigenic; AK and SCC) was associated with a statistically significant increase( p=<0.05) in: 1) CD20+ B lymphocytes (0.0±0.0 vs. 3.1 ± 0.5 vs. 7.5 ± 0.3 vs. 14.5 ± 5.5 ; 2) CD68 histiocytes (4.0±1.0 vs. 26.5 ± 3.9 vs. 23 ± 1.9 vs. 41.3 ± 6.8, ); 3) CD3+ T lymphocytes (3.0±1.1 vs. 58.3 ± 10.3 vs. 54.5 ± 0.2 vs. 41.0 ± 16.0); and 4) TIA-1+ cytotoxic T cells (1.8±0.4 vs. 2.9 ± 0.7 vs. 9.6 ± 1.1 vs. 13.7 ± 5.2). CONCLUSIONS: The increase in the number of infiltrating mononuclear cells in all pathologic lesions compared to normal skin may reflect increased antigenicity of the lesional cells. Both humoral and cell mediated immunity are involved in these lesions.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.