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Research Paper
C-Raf-1 Protein Kinase Is Not Essential for Ras Transformation of Mouse Embryo Fibroblasts
Anjali K. Gupta, Eric J. Bernhard, Vincent J. Bakanauskas,George J. Cerniglia, Ruth J. Muschel and Gillies McKenna
volume 2 | issue 1
January/February 2003Pages: 073-075
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Transfection of primary cells with mutated oncogenic ras plus a cooperating oncogene such as myc results in the acquisition of the transformed cell phenotype. The pathways downstream of Ras that are required for transformation are an active topic of research. The Raf-MEKK-MAP kinase pathway is triggered by activation of Ras and thought to be important in Ras transformation of rodent fibroblasts. To further explore the involvement of this pathway, fibroblasts from homozygous knock out c-Raf-1 mouse embryos (20 KO) and wild-type c-Raf-1 mouse embryos (16 WT) were transfected with H-ras and mycV. The resulting cell line derived from the knock out cells grew slower both in tissue culture and had a longer latency period as tumors than the transformed cell line from the wild-type cells. Both cell lines were however able to form tumors in nude mice. These results suggest that c-Raf-1 is not required for Ras transformation in this system. Key Words: c-Raf-1, Ras, Transformation
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.