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Research Paper
Increased Growth Rate, Delayed Senescense and Decreased Serum Dependence Characterize Cables-Deficient Cells
Sandra D. Kirley, Bo R. Rueda, Daniel C. Chung and Lawrence R. Zukerberg
volume 4 | issue 6
june 2006Pages: 654-658
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Cables (Cables1), a recently described growth suppressor protein that maps to human chromosome 18q 11-12, is lost in many primary colon, lung, and gynecological malignancies. Cultured cell lines that over-express Cables show a reduction in cell proliferation and Cables-/- mice are viable with normal embryonic development. Since Cables expression is lost in primary human tumors and over-expression of Cables suggests a role in growth suppression, we investigated growth properties of primary mouse embryonic fibroblasts (MEFs) from Cables-/- and Cables+/+ mice. Cables-/- MEFs exhibited a doubling time of 43-45 hours compared to 73-75 hours for the Cables+/+ MEFs. Similarly, Cables-/- MEFs show a delayed onset of senescence and extension of lifespan, while the Cables+/+ MEFs ceased proliferating after eight cumulative population doublings. Cables-/- MEFs were able to proliferate in low serum concentrations. These data provide convincing evidence that Cables plays a role in the regulation of cell proliferation and survival.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.