Authors: Alessandra Canazza, Chiara Calatozzolo, Luisa Fumagalli, Achille Bergantin, Francesco Ghielmetti, Laura Fariselli, Danilo Croci, Andrea Salmaggi and Emilio Ciusani

Alessandra Canazza

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Chiara Calatozzolo

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Luisa Fumagalli

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Achille Bergantin

Cyberknife Unit, Centro Diagnostico Italiano, Milan, Italy

Francesco Ghielmetti

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Laura Fariselli

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Danilo Croci

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Andrea Salmaggi

Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

Emilio Ciusani

Corresponding author: eciusani@istituto-besta.it
Fondazione IRCCS Istituto Neurologico “C. Besta”, Milano, Italy

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Abstract:

A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8Gy was delivered intermittently over a period of 40 minutes. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF-B at four days (Real time PCR), β1-integrin at 4-5 days (real-time PCR and western blot) and no elevation in phosphorylated AKT at days 4 and 5 (western blot). Our data suggests that glioma cell invasion as well as elevations of TGF-B and β1-integrin are associated with IIR and not CIR.