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Research Paper
Mutations in the BRCT Domain Confer Temperature Sensitivity to BRCA1 in Transcription Activation
Marcelo A. Carvalho, Blase Billack, Emily Chan, Terri Worley, Charmagne Cayanan and Alvaro A. Monteiro
volume 1 | issue 5
September/October 2002Pages: 502-508
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BRCA1 is a tumor suppressor gene and germ line mutations account for the majority of familial cases of breast and ovarian cancer. There is mounting evidence that BRCA1 functions in DNA repair and transcriptional regulation. A major hurdle to dissect the role of BRCA1 is the lack of molecular reagents to carry out biochemical and genetic experiments. Therefore, we used random mutagenesis of the C-terminus of BRCA1 (aa 1560–1863) to generate temperature-sensitive (TS) mutants in transcription activation. We obtained 11 TS mutants in transcription that localized primarily to the hydrophobic core of the BRCT-N domain of BRCA1. One of the mutants, H1686Q, also displayed temperature-dependent transcription activation in human cells. These conditional mutants represent valuable tools to assess the role of BRCA1 in transcription activation.
Key Words:
BRCA1, Yeast, Yranscription, Temperature- sensitive mutants, BRCT domain
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.