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Research Paper
Antitumor Effect of Valorphin In Vitro and In Vivo: Combined Action with Cytostatic Drugs
Elena Yu. Blishchenko, Olga V. Sazonova, Olga A. Kalinina, Ekaterina V. Moiseeva, Arpad A. Vass, Andrey A. Karelin, Vadim T. Ivanov
volume 4 | issue 1
january 2005Pages: 118-124
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The action of the cytostatic drugs (epirubicin and vincristine) in combination with the endogenous antiproliferative β-hemoglobin fragment (33-39), valorphin, was studied in tumor (L929 and A549) cell cultures, primary culture of murine bone marrow cells and in murine model of breast carcinoma in vivo. Simultaneous application of 1 然 valorphin and 1 然 epirubicin, in vitro, did not result in an additive suppressive effect on cell culture growth. Additive effects were achieved with alternating applications of the peptide and the drugs, namely, 0.5 然 (but not 1 然) epirubicin added 24 h prior to 1 然 valorphin; 1 然 valorphin added 48 h prior to 0.1 然 epirubicin, or 0.1 然 vincristine, or 0.05 然 vincristine, which resulted in 100% cell death in the both series with vincristine and up to 78% cell biomass reduction in the experiments with epirubicin. In the in vivo model (female BLRB mice with subcutaneously inoculated syngeneic mammary carcinoma), simultaneous treatment with 25 mg/m2 epirubicin and 1 mg/kg valorphin resulted in 42% of tumor growth inhibition, as compared with the negative control group and 22% inhibition as compared with the epirubcin-treated group (at 20th day of treatment). Survival was significantly improved (69% compared to 39% in the group treated with epirubicin only) at day 26 after the treatment beginning.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.