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Research Paper
DNA Ploidy and S Phase Fraction of Breast and Ovarian Tumor Cells Treated with a Natural Anthracycline Analog (Aloin)
Amr Y. Esmat, Shadia M. El-Gerzawy, Amira Rafaat
volume 4 | issue 1
january 2005Pages: 108-112
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DNA ploidy and S phase fraction analysis by flow cytometry on breast and ovarian tumor cells continuously exposed to aloin, a natural anthraquinone, at two concentrations (20-60 µg/ml) was done. Untreated breast and ovarian tumor cells (control) showed an aneuploid pattern, with a mean DNA index of 2.10± 0.10 and S phase fraction of 28.46± 1.5 and 17.40± 0.75%, respectively. Treatment of breast and ovarian tumor cells with aloin showed a persistent aneuploid pattern and a significantly dose-dependent increase in the percentage of S phase fraction and in the proportion of cells cycling at a higher ploidy level (>G2M). The polyploidization indicates that aloin does not inhibit initiation of DNA synthesis and that cells replicated a full complement of DNA but had difficulty in M phase.
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDFIf the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.