• Comprehensive histological evaluation of bone implants
  • Grafting of a model protein on lactide and caprolactone based biodegradable films for biomedical applications
  • Characterization of a bioactive fiber scaffold with entrapped HUVECs in coaxial electrospun core-shell fiber

 Comprehensive histological evaluation of bone implants

Claudia Rentsch, Wolfgang Schneiders, Suzanne Manthey, Barbe Rentsch and Stephan Rammelt


To investigate and assess bone regeneration in sheep in combination with new implant materials classical histological staining methods as well as immunohistochemistry may provide additional information to standard radiographs or computer tomography. Available published data of bone defect regenerations in sheep often present none or sparely labeled histological images. Repeatedly, the exact location of the sample remains unclear, detail enlargements are missing and the labeling of different tissues or cells is absent. The aim of this article is to present an overview of sample preparation, staining methods and their benefits as well as a detailed histological description of bone regeneration in the sheep tibia. General histological staining methods like hematoxylin and eosin, Masson-Goldner trichrome, Movat’s pentachrome and alcian blue were used to define new bone formation within a sheep tibia critical size defect containing a polycaprolactone-co-lactide (PCL) scaffold implanted for 3 months (n = 4). Special attention was drawn to describe the bone healing patterns down to cell level. Additionally one histological quantification method and immunohistochemical staining methods are described.

 Grafting of a model protein on lactide and caprolactone based biodegradable films for biomedical applications

Aitor Larrañaga, Andrée-Anne Guay-Bégin, Pascale Chevallier, Gad Sabbatier, Jorge Fernández, Gaétan Laroche and Jose-Ramon Sarasua

 Characterization of a bioactive fiber scaffold with entrapped HUVECs in coaxial electrospun core-shell fiber

Hui Ying Ang, Scott Alexander Irvine, Ron Avrahami, Udi Sarig, Tomer Bronshtein, Eyal Zussman, Freddy Yin Chiang Boey, Marcelle Machluf and Subbu S Venkatraman

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Editor-in-Chief

Pedro L. Granja
INEB—Instituto de
Engenharia Biomédica
Universidade do Porto
Porto, Portugal