Engineering influenza viral vectors

Junwei Li, Maria T. Arévalo, * Mingtao Zeng     Pages 9 - 14
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Submitted
July 16, 2012
Revised
August 22, 2012
Accepted
August 23, 2012
Published Online
August 24, 2012

Keywords: gene delivery, gene therapy, influenza virus, microRNA delivery, pseudo-typed virus, reverse genetics, vaccine, viral vector

  Abstract: The influenza virus is a respiratory pathogen with a negative-sense, segmented RNA genome. Construction of recombinant influenza viruses in the laboratory was reported starting in the 1980s. Within a short period of time, pioneer researchers had devised methods that made it possible to construct influenza viral vectors from cDNA plasmid systems. Herein, we discuss the evolution of influenza virus reverse genetics, from helper virus-dependent systems, to helper virus-independent 17-plasmid systems, and all the way to 3- and 1- plasmid systems. Successes in the modification of different gene segments for various applications, including vaccine and gene therapies are highlighted.

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Engineering influenza viral vectors
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