GFP fluorescence quenching
It is well established that GFP fluorescence is quenched in the acidic environment of the lysosome. However, does anyone know if this is the case as well in fixed cells? Would the fixation method influence this (methanol vs formaldehyde)?
Thank you
October 20th, 2012 at 3:43 am
Longer fixation time with formalin say 30 min reduces fluorescence.
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