Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice

Akiko Kuma and Noboru Mizushima

volume 4 | issue 1

1 January 2008
Pages: 61 - 62

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GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP-LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3^tg/tg) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.

Authors

Akiko Kuma

Department of Physiology and Cell Biology; Tokyo Medical and Dental University; Tokyo Japan; Genome Research Institute; University of Cincinnati; Cincinnati, Ohio USA

Noboru Mizushima

Department of Physiology and Cell Biology; Tokyo Medical and Dental University; Tokyo Japan

We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link: