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Research Paper
Coronavirus Replication Does Not Require the Autophagy Gene ATG5
Zijiang Zhao, Larissa B. Thackray, Brian C. Miller, Teresa M. Lynn, Michelle M. Becker, Eric Ward, Noboru Mizushima, Mark R. Denison and Herbert W. Virgin
volume 3 | issue 6
November/December 2007Pages: 581 - 585
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Macroautophagy (herein autophagy) is a cellular process, requiring ATG5, by which cells deliver double membrane-bound packets containing cytoplasm or cytoplasmic organelles to the lysosome. This process has been reported in some cases to be antiviral, while in other cases it has been reported to be required for efficient viral replication or release. A role for autophagy in RNA virus replication has been an attractive hypothesis because of the association of RNA virus replication with complex membrane rearrangements in the cytoplasm that can generate opposed double membranes. In this study we demonstrate that ATG5 is not required for murine hepatitis virus (MHV) replication in either bone marrow derived macrophages (BMMφ) lacking ATG5 by virtue of Cre-recombinase mediated gene deletion or primary low passage murine ATG5-/- embryonic fibroblasts (pMEFs). We conclude that neither ATG5 nor an intact autophagic pathway are required for MHV replication or release.
Authors
Zijiang Zhao
Washington University School of Medicine
Larissa B. Thackray
Washington University School of Medicine
Brian C. Miller
Washington University School of Medicine
Teresa M. Lynn
Washington University School of Medicine
Michelle M. Becker
Vanderbilt University School of Medicine
Eric Ward
Vanderbilt University School of Medicine
Noboru Mizushima
Department of Physiology and Cell Biology; Tokyo Medical and Dental University; Tokyo Japan
Mark R. Denison
Vanderbilt University School of Medicine
Herbert W. Virgin
Washington University School of Medicine
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.