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Sequestration Revisited: Integrating Traditional Electron Microscopy, De Novo Assembly and New Results
Attila L. Kovács, Zsolt Pálfia, Gábor Réz, Tibor Vellai and János Kovács
volume 3 | issue 6
November/December 2007Pages: 655 - 662
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Electron microscopy analysis of the autophagic sequestration membrane (SM) in various metazoan cell types after different fixation methods shows that: (1) the growing SM cannot derive from preformed rough surfaced endoplasmic reticulum (RER) membranes by transformation; (2) the empty cleft between the two layers of the SM after aldehyde fixation is an artifact of sample preparation; (3) the SM emerges from and grows de novo in cytoplasmic areas where membranous precursors cannot be identified by traditional electron microscopy; (4) the growing SM consists of two tightly packed membrane layers with a sharp bend at the edge; (5) changes in the environment of the growing SM participate in the determination of the size and shape of the autophagosome.
Authors
Attila L. Kovács
Eötvös Loránd University
Zsolt Pálfia
Eötvös Loránd University
Gábor Réz
Eötvös Loránd University
Tibor Vellai
Eötvös Loránd University
János Kovács
Eötvös Loránd University
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.