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Article Addendum
mTOR Kinase and the Regulatory Subunit of Protein Kinase A (PRKAR1A) Spatially and Functionally Interact during Autophagosome Maturation
Manos Mavrakis, Jennifer Lippincott-Schwartz, Constantine A. Stratakis and Ioannis Bossis
volume 3 | issue 2
March/April 2007Pages: 151 - 153
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The regulatory subunit 1-alpha (RI-alpha) of protein kinase A (PKA) and the mTOR kinase are involved in a common pathway regulating mammalian autophagy. RI-alpha was found to localize on Rab7-positive late endosomes and on LC3-positive autophagosomal membranes in cultured cells. RI-alpha was also shown to physically interact with mTOR kinase and affect its phosphorylation and activity.1 In this addendum, we further explore the subcellular distribution of mTOR related to RI_ and LC3. We present experiments showing that mTOR colocalizes with RI-alpha-, Rab7- and LC3-positive membranes in cultured cells. Because RIa regulates the phosphorylation and activity of mTOR kinase, which we now show localizes on autophagosomal membranes, the possibility emerges that the RI-alpha-mTOR complex acts at the level of autophagosome maturation.
Addendum to:
Depletion of Type IA Regulatory Subunit (RI-alpha) of Protein Kinase A (PKA) in Mammalian Cells and Tissues Activates mTOR and Causes Autophagic Deficiency
M. Mavrakis, J. Lippincott-Schwartz, C.A. Stratakis and I. Bossis
Hum Mol Genet 2006; 15:2962-71
Authors
Manos Mavrakis
NIH/NICHD
Jennifer Lippincott-Schwartz
NIH/NICHD
Constantine A. Stratakis
NIH/NICHD
Ioannis Bossis
NIH/NICHD
We now provide open access to journal articles published online for one year or more. This article may be downloaded at the following link:
Download PDF If the document does not open, please right-click on the link (control-click on a Macintosh) and select the option to save the file to disk.