Long time-lapse imaging reveals unique features of PARK2/Parkin-mediated mitophagy in mature cortical neurons
Volume 8, Issue 6
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Pages 976 - 978http://dx.doi.org/10.4161/auto.20218
: PARK2, Parkin, autophagosome, autophagy, depolarization, lysosome, mitochondria, mitochondrial membrane potential, mitochondrial mobility, mitochondrial quality control, neuronal mitophagy
Authors: Qian Cai, Hesham Mostafa Zakaria and Zu-Hang Sheng View affiliations
Proper degradation of aged and damaged mitochondria through mitophagy is essential to ensure mitochondrial integrity and function. Translocation of PARK2/Parkin onto damaged mitochondria induces mitophagy in many non-neuronal cell types. However, direct evidence showing PARK2-mediated mitophagy in mature neurons is controversial, leaving unanswered questions as to how, where, and by what time course PARK2-mediated mitophagy occurs in neurons following mitochondrial depolarization. We applied long time-lapse imaging in live mature cortical neurons to monitor the slow but dynamic and spatial PARK2 translocation onto damaged mitochondria and subsequent degradation through the autophagy-lysosomal pathway. In comparison with non-neuronal cells, our study reveals unique features of PARK2-mediated mitophagy in mature neurons, which will advance our understanding of pathogenesis of several major neurodegenerative diseases characterized by damaged mitochondria or a dysfunctional autophagy-lysosomal system.
Autophagic Punctum to:
Q Cai, HM Zakaria, A Simone, Z-H Sheng. Spatial Parkin translocation and degradation of damaged mitochondria via mitophagy in live cortical neurons. Curr Biol 2012; 22: 545-52
PMID: 22342752 DOI: 10.1016/j.cub.2012.02.005
Received: March 19, 2012; Accepted: March 30, 2012; Published Online: June 1, 2012
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