Autophagic Punctum

Autophagosome biogenesis requires SNAREs

Volume 7, Issue 12   December 2011
Pages 1570 - 1572
http://dx.doi.org/10.4161/auto.7.12.18001
Keywords: Atg9, SNARE, autophagy, lysosome, phagophore assembly site, tubulovesicular clusters, vacuole
Authors: Usha Nair and Daniel J. Klionsky

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Abstract:
We recently showed that phagophore biogenesis requires SNAREs. Our data indicate that the exocytic Q/t-SNAREs Sso1/2 and Sec9 are required for one of the earliest steps in autophagosome biogenesis, the homotypic fusion of Atg9-containing vesicles. We propose that this step precedes the formation of Atg9-containing tubulovesicular clusters (TVCs) that is a key step in perivacuolar, phagophore assembly. We also found that the endosomal Q/t-SNARE Tlg2 and the R/v-SNAREs Sec22 and Ykt6 interact with Sso1-Sec9, and are required for normal Atg9 trafficking. Thus, autophagosome biogenesis appears to involve multiple SNARE-mediated fusion events. These findings provide novel insights into the mechanism of autophagosome construction.

Autophagic Punctum to:
SM Douglas, I Bachelet, GM Church. A logic-gated nanorobot for targeted transport of molecular payloads. Science 2012; 335: 831-4
PMID: 22344439 DOI: 10.1126/science.1214081

Received: August 30, 2011; Accepted: September 6, 2011

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