Development of Adeniviral Vectors for Gene Therapy
Dai Katayose, Prem Seth
In recent years adenoviruses have been extensively used as vectors to deliver foreign genome into mammalian cells (reviewed in refs. 1-5). Adenoviruses have certain features, which make them attractive vectors for gene transfer to target cells. Some of these properties include their ability to infect a broad range of cell types, including dividing as well as nondividing cells, the ease with which adenovirus genome can be manipulated, and the ability to obtain high titers. There are essentially two ways by which adenoviruses can be used to deliver foreign DNA into cells. One approach utilizes introduction of the foreign cDNAs into the adenovirus genome, resulting in recombinant adenoviruses containing the gene of choice. In the second approach, the ability of adenovirus to enter the cytosol by disrupting the endosome membrane is used to enhance the delivery of the foreign DNA into the cells. In this chapter we describe these two methods of adenovirally-mediated DNA delivery.