The RNA infrastructure connects RNA‑based functions. With transcription‑ to‑translation processing forming the core of the network, we can visualise how RNA‑based regulation, cleavage and modification are the backbone of cellular function. The key to interpreting the RNA‑infrastructure is in understanding how core RNAs (tRNA, mRNA and rRNA) and other ncRNAs operate in a spatial‑temporal manner, moving around the nucleus, cytoplasm and organelles during processing, or in response to environmental cues. This chapter summarises the concept of the RNA‑infrastructure, and highlights examples of RNA‑based networking within prokaryotes and eukaryotes. It describes how transcription‑to‑translation processes are tightly connected, and explores some similarities and differences between prokaryotic and eukaryotic RNA networking.