Bacteria Mediated Gene Therapy Strategies
Sophie Conchon and Georges Vassaux
The recent discovery that genes carried by bacterial vectors can be functionally transferred to mammalian cells has led to the utilization of various bacterial strains in gene therapy. Genetically modified, nonpathogenic bacteria that have been used include attenuated strains of Salmonella, Shigella, Listeria, Yersinia, Clostridium or Bifidobacterium. Their potential as tumor‑targeting agents, as well as their ability to deliver therapeutic molecules have been demonstrated in vitro and in vivo in experimental models. Therapeutic benefits have been observed in immunotherapy against cancer, vaccination against infectious diseases and topical delivery of immunomodulatory cytokines in inflammatory bowel disease. The studies presented in this review show that bacteria can be considered as a promising therapeutic vector against various pathologies.