Advances in Insect-Based Cell-Free Protein Expression
Uritza von Groll, Stefan Kubick, Helmut Merk, Wolfgang Stiege and Frank Schäfer
Cell-free protein expression systems are becoming more widely used as they allow fast synthesis of recombinant proteins and an easy manipulation of reaction conditions. Here, we report on recent advances of a eukaryotic system based on extracts derived from Spodoptera frugiperda insect cells. We demonstrate the use of PCR products as DNA templates for protein production and how this application speeds up in vitro protein expression and potentially cell-based production processes. We also show the expression of a variety of functionally active eukaryotic proteins, including kinases and membrane proteins. Finally, we compare the efficiency of this improved insect-based in vitro protein expression system to traditionally used eukaryotic cell-free extracts, such as rabbit reticulocyte lysate.