The PURE system: A Minimal Cell-Free Translation System
Bei-Wen Ying, Yoshihiro Shimizu and Takuya Ueda
A reconstituted protein synthesis system, comprising a minimal set of components for the production of active proteins, is described. The system consists of 32 purified soluble factors from Escherichia coli together with other biochemical components, and is designated as the PURE (Protein synthesis Using Recombinant Elements) system. The PURE system can be conveniently used for both the production of functional target proteins and for investigation of protein translation and maturation processes. The synthesis of various proteins, including model proteins, single-chain antibodies, multiple-domain proteins, etc., is examined. Moreover, biochemical applications for protein folding, particularly chaperone-mediated processes are further discussed.